最近对专业文章翻译突然没感觉了，具体情况见下文：
原文1：
Cell Culture
hMSCs(human marrow mesenchymal tem cells) were obtained from Prof. Lingsong Li(Peking University Stem cell Research Center,Beijing China).This is a well-characterize hMSC cell line. hMACs were cultured with 10% FBS in Alpha MEM complete media and were plated in a 25-cm2 flasks at a density of 10^4 cells/cm2 or into wells of 24-well plate at a density of 3000~4000 cells/cm2 and incubated at 37℃ in 5% CO2. After 72 hours, the meida were replace. At 80%~90% confluence, cells were harvested with 0.25% trypsin(Sigma) for 3 minutes at 37℃. Osteogenic differentiation was assessed by incubating hMSCs with Alpha MEM osteogenic-inducing media for 7,14 or 21 days with media changes twice weekly.
原文2：
Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease
Parkinson's disease is a widespread condition caused by the loss of midbrain neurons that synthesize the neurotransmitter dopamine. Cells derived from the fetal midbrain can modify the course of the disease, but they are an inadequate source of dopamine-synthesizing neurons because their ability to generate these neurons is unstable. In contrast, embryonic stem (ES) cells proliferate extensively and can generate dopamine neurons. If ES cells are to become the basis for cell therapies, we must develop methods of enriching for the cell of interest and demonstrate that these cells show functions that will assist in treating the disease. Here we show that a highly enriched population of midbrain neural stem cells can be derived from mouse ES cells. The dopamine neurons generated by these stem cells show electrophysiological and behavioral properties expected of neurons from the midbrain. Our results encourage the use of ES cells in cell-replacement therapy for Parkinson's disease.
译文1：
细胞培养
hMSCs(人类骨髓间充质干细胞)被李林松教授(北京大学干细胞研究中心，北京，中国)获取。这是个性质良好的hMSC细胞株。hMACs在含10% FBS的α-MEM完全培养基及25cm2瓶里以每平方厘米10^4个细胞或在含5% CO2的24口径培养皿中37℃以每平方厘米3000至4000个细胞的密度来培养。72小时后，更换培养基。达到80%~90%汇合状态时，用0.25%胰蛋白酶在37℃下处理3分钟，收获细胞。用α-MEM成骨质诱导培养基抚育hMSCs1~3周，每周更换两次培养基，hMSCs分化为成骨质。
译文2：
由胚胎干细胞生成的多巴胺能神经元在一个帕金森症的动物模型上起功效（译得暴烂的标题）
帕金森症是由合成神经递质多巴胺的中脑神经元的死亡而引起的一个分布广泛的症状。胎儿中脑细胞能缓解疾病的发生，但是它们不适合用于多巴胺合成，因为它们生成这些神经元的能力不稳定。与之对照的是，胚胎干细胞能大量增殖并能生成多巴胺能神经元。如果以胚胎干细胞为基础进行细胞治疗，我们必须开发出细胞增值法以用于增殖感兴趣的细胞及证明这些细胞能表现出辅助治疗疾病的功能。我们在此证明能从小鼠干细胞里获得非常丰富的中脑干细胞。由这些干细胞生成的多巴胺能神经元表现出中脑神经元的电生理学及活动特征。我们的试验结果给干细胞来进行帕金森症的细胞置换疗法带来了希望。（看完后只有一个晕字）
